› 6quy

Electron Microscopy
3.8Å resolution

NgCKK (N.Gruberi CKK) decorated 13pf taxol-GDP microtubule

Released:
DOI: 10.2210/pdb6quy/pdb
PDB 6quy coloured by chain and viewed from the front.
PDB 6quy coloured by chain and viewed from the side.
PDB 6quy coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural determinants of microtubule minus end preference in CAMSAP CKK domains.
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun 10 5236 (2019)
PMID: 31748546
Related structures: EMD-4644

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68363 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
CKK domain-containing protein Chain: W
Molecule details ›
Chain: W
Length: 187 amino acids
Theoretical weight: 20.88 KDa
Source organism: Naegleria gruberi
Expression system: Escherichia coli
UniProt:
  • Canonical: D2VJG4 (Residues: 621-788; Coverage: 21%)
Gene name: NAEGRDRAFT_50049
Sequence domains: Microtubule-binding calmodulin-regulated spectrin-associated
Tubulin beta chain Chains: G, H
Molecule details ›
Chains: G, H
Length: 444 amino acids
Theoretical weight: 49.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07437 (Residues: 1-444; Coverage: 100%)
Gene names: OK/SW-cl.56, TUBB, TUBB5
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GDP 2 x GDP
Ligand TA1 2 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4644
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy.
Luo et al. (2020)