Assemblies
Assembly Name:
DNA-directed RNA polymerase and RNA and DNA
Multimeric state:
hetero octamer
Accessible surface area:
160608.53 Å2
Buried surface area:
38016.9 Å2
Dissociation area:
1,307.5
Å2
Dissociation energy (ΔGdiss):
3.91
kcal/mol
Dissociation entropy (TΔSdiss):
12.52
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-119909
Macromolecules
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0A7Z4 (Residues: 1-329; Coverage: 100%)
Pfam:
- RNA polymerase Rpb3/Rpb11 dimerisation domain
- RNA polymerase Rpb3/RpoA insert domain
- Bacterial RNA polymerase, alpha chain C terminal domain
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Pfam:
- RNA polymerase beta subunit
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 3
- RNA polymerase beta subunit external 1 domain
- RNA polymerase Rpb2, domain 6
- RNA polymerase Rpb2, domain 7
- DNA-directed RNA polymerase beta subunit, bacterial-type
- RNA polymerase, beta subunit, protrusion
- DNA-directed RNA polymerase, subunit 2
- RNA polymerase Rpb2, domain 2
- RNA polymerase Rpb2, domain 2 superfamily
- RNA polymerase Rpb2, domain 3
- DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily
- DNA-directed RNA polymerase, beta subunit, external 1 domain
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain
- RNA polymerase Rpb2, OB-fold
- DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily
- RNA polymerase, beta subunit, conserved site
- RNA polymerase Rpb2, domain 7
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Pfam:
- RNA polymerase Rpb1, domain 1
- RNA polymerase Rpb1, domain 2
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
- RNA polymerase Rpb1, clamp domain superfamily
- DNA-directed RNA polymerase, subunit beta-prime, bacterial type
- RNA polymerase Rpb1, domain 1
- DNA-directed RNA polymerase, subunit beta-prime
- RNA polymerase, N-terminal
- RNA polymerase, alpha subunit
- RNA polymerase Rpb1, domain 3
- RNA polymerase Rpb1, domain 3 superfamily
- RNA polymerase Rpb1, funnel domain superfamily
- RNA polymerase Rpb1, domain 4
- RNA polymerase Rpb1, domain 5
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: RNA polymerase Rpb6
InterPro:
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Pfam: RNA polymerase Rpb6
InterPro:
Name:
DNA (30-MER)
Representative chains: N
Length: 31 nucleotides
Theoretical weight: 9.68 KDa
Representative chains: N
Length: 31 nucleotides
Theoretical weight: 9.68 KDa
Name:
DNA (39-MER)
Representative chains: T
Length: 39 nucleotides
Theoretical weight: 11.82 KDa
Representative chains: T
Length: 39 nucleotides
Theoretical weight: 11.82 KDa
Name:
RNA (5'-R(P*GP*AP*UP*GP*UP*GP*UP*GP*CP*U)-3')
Representative chains: R
Length: 10 nucleotides
Theoretical weight: 3.19 KDa
Representative chains: R
Length: 10 nucleotides
Theoretical weight: 3.19 KDa
