› 4ztd

X-ray diffraction
2.2Å resolution

Crystal Structure of Human PCNA in complex with a TRAIP peptide

Released:
DOI: 10.2210/pdb4ztd/pdb
PDB entry 4ztd coloured by chain, front view.
PDB entry 4ztd coloured by chain, side view.
PDB entry 4ztd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress.
Hoffmann S, Smedegaard S, Nakamura K, Mortuza GB, Räschle M, Ibañez de Opakua A, Oka Y, Feng Y, Blanco FJ, Mann M, Montoya G, Groth A, Bekker-Jensen S, Mailand N
J Cell Biol 212 63-75 (2016)
PMID: 26711499

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146053 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 253 amino acids
Theoretical weight: 27.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 2-254; Coverage: 97%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
E3 ubiquitin-protein ligase TRAIP Chains: D, E
Molecule details ›
Chains: D, E
Length: 12 amino acids
Theoretical weight: 1.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BWF2 (Residues: 459-469; Coverage: 2%)
Gene names: RNF206, TRAIP, TRIP
ALA-GLY-ALA-GLY-ALA Chain: F
Molecule details ›
Chain: F
Length: 5 amino acids
Theoretical weight: 345 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P65
Unit cell:
a: 84.36Å b: 84.36Å c: 210.862Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Cell cycle arrest through indirect transcriptional repression by p53: I have a DREAM.
Engeland K. (2018)
11 more

6 mentions without citation

Human PCNA Structure, Function and Interactions.
González-Magaña et al. (2020)
5 more