PDBe 4pki

X-ray diffraction
2.3Å resolution

Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Oryctolagus cuniculus
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Gelsolin; Tropomodulin-1 Chain: G
Molecule details ›
Chain: G
Length: 324 amino acids
Theoretical weight: 36.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
  • Canonical: P28289 (Residues: 160-349; Coverage: 53%)
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Gene names: D9S57E, GSN, TMOD, TMOD1
Sequence domains: Gelsolin repeat
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 69.393Å b: 81.211Å c: 170.708Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.168 0.2
Expression system: Escherichia coli BL21(DE3)