› 3kyn

X-ray diffraction
2.4Å resolution

Crystal structure of HLA-G presenting KGPPAALTL peptide

Released:
DOI: 10.2210/pdb3kyn/pdb
PDB entry 3kyn coloured by chain, front view.
PDB entry 3kyn coloured by chain, side view.
PDB entry 3kyn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure and stability of the monomorphic HLA-G are influenced by the nature of the bound peptide.
Walpole NG, Kjer-Nielsen L, Kostenko L, McCluskey J, Brooks AG, Rossjohn J, Clements CS
J Mol Biol 397 467-80 (2010)
PMID: 20122941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-148134 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, alpha chain G Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17693 (Residues: 26-299; Coverage: 87%)
Gene names: HLA-6.0, HLA-G, HLAG
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
KGPPAALTL peptide Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 868 Da

Ligands and Environments

2 bound ligands:
Ligand CO 1 x CO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 76.874Å b: 76.874Å c: 151.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.217 0.299
Expression system: Escherichia coli

Quick links

Citations

7 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
6 more

2 mentions without citation

HLA-G and the MHC Cusp Theory.
Scavuzzi et al. (2022)
1 more