X-ray diffraction
3.21Å resolution

Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1


Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-153636 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breast cancer type 1 susceptibility protein Chains: A, B, C, D, E, F, G, X
Molecule details ›
Chains: A, B, C, D, E, F, G, X
Length: 235 amino acids
Theoretical weight: 26.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
Acetyl-CoA carboxylase 1 Chains: H, I, J, K, L, M, N, O
Molecule details ›
Chains: H, I, J, K, L, M, N, O
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided
  • Canonical: Q13085 (Residues: 1258-1270; Coverage: 1%)
Gene names: ACAC, ACACA, ACC1, ACCA

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 83.33Å b: 181.513Å c: 194.643Å
α: 90° β: 90° γ: 90°
R R work R free
0.26 0.257 0.308
Expression systems:
  • Escherichia coli
  • Not provided