› 3coj

X-ray diffraction
3.21Å resolution

Crystal Structure of the BRCT Domains of Human BRCA1 in Complex with a Phosphorylated Peptide from Human Acetyl-CoA Carboxylase 1

Released:
DOI: 10.2210/pdb3coj/pdb
PDB entry 3coj coloured by chain, front view.
PDB entry 3coj coloured by chain, side view.
PDB entry 3coj coloured by chain, top view.
Source organisms:
Primary publication:
Structural evidence for direct interactions between the BRCT domains of human BRCA1 and a phospho-peptide from human ACC1.
Shen Y, Tong L
Biochemistry 47 5767-73 (2008)
PMID: 18452305

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153636 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breast cancer type 1 susceptibility protein Chains: A, B, C, D, E, F, G, X
Molecule details ›
Chains: A, B, C, D, E, F, G, X
Length: 235 amino acids
Theoretical weight: 26.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
Acetyl-CoA carboxylase 1 Chains: H, I, J, K, L, M, N, O
Molecule details ›
Chains: H, I, J, K, L, M, N, O
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q13085 (Residues: 1258-1270; Coverage: 1%)
Gene names: ACAC, ACACA, ACC1, ACCA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 8 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 83.33Å b: 181.513Å c: 194.643Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.257 0.308
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
10 more

9 mentions without citation

A guide for functional analysis of BRCA1 variants of uncertain significance.
Millot et al. (2012)
8 more