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X-ray diffraction
2.2Å resolution

mutated b-glucosidase A from Paenibacillus polymyxa showing increased stability

Released:
Source organism: Paenibacillus polymyxa
Entry authors: Isorna P, Polaina J, Sanz-Aparicio J

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase A Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 51.51 KDa
Source organism: Paenibacillus polymyxa
Expression system: Escherichia coli
UniProt:
  • Canonical: P22073 (Residues: 2-448; Coverage: 100%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: I422
Unit cell:
a: 136.416Å b: 136.416Å c: 173.341Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.227
Expression system: Escherichia coli