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CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE

X-ray diffraction
2.2Å resolution
PDB entry 1lf9 coloured by chain, front view.
PDB entry 1lf9 coloured by chain, side view.
PDB entry 1lf9 coloured by chain, top view.
Source organism: Thermoanaerobacterium thermosaccharolyticum
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucoamylase
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Multimeric state: monomeric
Accessible surface area: 22617.08 Å2
Buried surface area: 1341.26 Å2
Dissociation area: 453.27 Å2
Dissociation energy (ΔGdiss): -2.93 kcal/mol
Dissociation entropy (TΔSdiss): 6.02 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131441
Assembly 2
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Assembly Name: Glucoamylase
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Multimeric state: monomeric
Accessible surface area: 22571.24 Å2
Buried surface area: 1336.12 Å2
Dissociation area: 451.58 Å2
Dissociation energy (ΔGdiss): -2.59 kcal/mol
Dissociation entropy (TΔSdiss): 6.02 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131441

Macromolecules

Glucoamylase
Chains: A, B
Length: 684 amino acids
Theoretical weight: 76.59 KDa
Source organism: Thermoanaerobacterium thermosaccharolyticum
UniProt:
  • Canonical: O85672 (Residues: 18-141, 142-695; Coverage: 100%)
Gene name: ga1
Pfam:
InterPro:
CATH:
SCOP:
1,4-alpha-D-glucan glucohydrolase in PDB entry 1lf9, assembly 1, front view.
1,4-alpha-D-glucan glucohydrolase in PDB entry 1lf9, assembly 1, side view.
1,4-alpha-D-glucan glucohydrolase in PDB entry 1lf9, assembly 1, top view.
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