› 5zk5

X-ray diffraction
2.25Å resolution

Stapled-peptides tailored against initiation of translation

Released:
DOI: 10.2210/pdb5zk5/pdb
PDB entry 5zk5 coloured by chain, front view.
PDB entry 5zk5 coloured by chain, side view.
PDB entry 5zk5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights reveal a recognition feature for tailoring hydrocarbon stapled-peptides against the eukaryotic translation initiation factor 4E protein.
Lama D, Liberatore AM, Frosi Y, Nakhle J, Tsomaia N, Bashir T, Lane DP, Brown CJ, Verma CS, Auvin S
OpenAccess logo Chem Sci 10 2489-2500 (2019)
PMID: 30881679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139175 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06730 (Residues: 28-217; Coverage: 88%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Eukaryotic translation initiation factor 4 gamma 1 Chain: B
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 2.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04637 (Residues: 609-623; Coverage: 1%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

1 bound ligand:
Ligand MGT 1 x MGT
1 modified residue:
Ligand MK8 2 x MK8

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 37.48Å b: 65.09Å c: 76.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.198 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Bioinformatics and Biosimulations as Toolbox for Peptides and Peptidomimetics Design: Where Are We?
D'Annessa et al. (2020)
3 more

1 mention without citation

Structural insights reveal a recognition feature for tailoring hydrocarbon stapled-peptides against the eukaryotic translation initiation factor 4E protein.
Lama et al. (2019)