2c5s

X-ray diffraction
2.5Å resolution

Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-182564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable tRNA sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.97 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q81KU0 (Residues: 1-403; Coverage: 100%)
Gene names: BAS4545, BA_4899, GBAA_4899, thiI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3221
Unit cell:
a: 81.047Å b: 81.047Å c: 140.893Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.227 0.278
Expression system: Escherichia coli BL21