Thil, AANH domain (IPR020536)

Short name: ThiI_AANH

Overlapping homologous superfamilies

Domain relationships



tRNA sulfurtransferase ThiI catalyses the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor [PMID: 11443125, PMID: 16855700, PMID: 18604845]. In some bacteria, ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety in the thiamine biosynthesis pathway [PMID: 9209060].

Three domains of ThiI are essential for the thiolation of tRNA: a THUMP domain that binds tRNA (see IPR004114) [PMID: 16343540], an AANH domain that activates the uridine residue by adenylylation [PMID: 18604845], and a rhodanese domain that transfers sulfur to the activated uridine residue [PMID: 11443125]. Neither the THUMP nor the AANH domain of ThiI are essential for thiamine synthesis, only the rhodanese domain is necessary for synthesis of the thiazole moiety of thiamine [PMID: 21724998].

This entry represents the AANH domain.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0004810 tRNA adenylyltransferase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.