PDBsum entry 1vw3

Ribosome

PDB id: 1vw3

Contents

Protein chains

295 a.a.

249 a.a.

239 a.a.

DNA/RNA

Metals

_MG ×109

_NA

Obsolete entry

PDB id:

1vw3

Name:

Ribosome

Title:

Structure of the yeast mitochondrial large ribosomal subunit

Structure:

21s ribosomal RNA. Chain: a. 54s ribosomal protein rml2, mitochondrial. Chain: b. Synonym: l2. 54s ribosomal protein l9, mitochondrial. Chain: c. Synonym: yml9. 54s ribosomal protein yml6, mitochondrial.

Source:

Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Organism_taxid: 4932

Authors:

A.Amunts,A.Brown,X.C.Bai,J.L.Llacer,T.Hussain,P.Emsley,F.Long, G.Murshudov,S.H.W.Scheres,V.Ramakrishnan

Key ref:

A.Amunts et al. (2014). Structure of the yeast mitochondrial large ribosomal subunit. Science, 343, 1485-1489. PubMed id: 24675956 DOI: 10.1126/science.1249410

Date:

22-Jan-14 Release date: 09-Apr-14

Protein chain

P32611  (RML2_YEAST) -  54S ribosomal protein RML2, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 393 a.a.

Struc: 295 a.a.

Protein chain

P31334  (RM09_YEAST) -  54S ribosomal protein L9, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 269 a.a.

Struc: 249 a.a.

Protein chain

P51998  (RL4P_YEAST) -  54S ribosomal protein YmL6, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 286 a.a.

Struc: 239 a.a.

DNA/RNA chain

G-U-A-G-A-A-U-A-A-U-A-G-A-U-U-U-G-A-A-A-U-A-U-U-U-A-U-U-A-U-A-U-A-G-A-U-U-U-A- ... 2713 bases

DOI no: 10.1126/science.1249410

Science 343:1485-1489 (2014)

PubMed id: 24675956

Structure of the yeast mitochondrial large ribosomal subunit.

A.Amunts, A.Brown, X.C.Bai, J.L.Llácer, T.Hussain, P.Emsley, F.Long, G.Murshudov, S.H.Scheres, V.Ramakrishnan.

ABSTRACT

Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.