› 8jru

Electron Microscopy
3.5Å resolution

Cryo-EM structure of the glucagon receptor bound to beta-arrestin 1 in ligand-free state

Released:
DOI: 10.2210/pdb8jru/pdb
PDB entry 8jru coloured by chain, front view.
PDB entry 8jru coloured by chain, side view.
PDB entry 8jru coloured by chain, top view.
Source organisms:
Primary publication:
Tail engagement of arrestin at the glucagon receptor.
Chen K, Zhang C, Lin S, Yan X, Cai H, Yi C, Ma L, Chu X, Liu Y, Zhu Y, Han S, Zhao Q, Wu B
Nature (2023)
PMID: 37558880
Related structures: EMD-36606

Function and Biology Details

Reaction catalysed: 
Degradation of blood coagulation factors Va and VIIIa.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-239641 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Activation peptide; Glucagon receptor; Hemagglutinin; Vasopressin V2 receptor Chain: R
Molecule details ›
Chain: R
Length: 478 amino acids
Theoretical weight: 54.31 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03435 (Residues: 1-16; Coverage: 0%)
  • Canonical: P04070 (Residues: 205-216; Coverage: 3%)
  • Canonical: P47871 (Residues: 27-432; Coverage: 90%)
  • Canonical: P30518 (Residues: 343-371; Coverage: 8%)
Gene names: ADHR, AVPR2, DIR, DIR3, GCGR, HA, PROC, V2R
Sequence domains:
Beta-arrestin-1 Chains: A, H, L
Molecule details ›
Chains: A, H, L
Length: 627 amino acids
Theoretical weight: 69.17 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P17870 (Residues: 1-379; Coverage: 91%)
Gene name: ARRB1
Sequence domains:
Nanobody 32 Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia phage EcSzw-2
Expression system: Escherichia coli BL21

Ligands and Environments

1 bound ligand:
Ligand PIO 1 x PIO
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-36606
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21

Quick links

Citations

1 mention without citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)