› 8dr8

Electron Microscopy
3.04Å resolution

LRRC8A:C conformation 2 (oblong) top mask

Released:
DOI: 10.2210/pdb8dr8/pdb
PDB entry 8dr8 coloured by chain, front view.
PDB entry 8dr8 coloured by chain, side view.
PDB entry 8dr8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels.
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023)
PMID: 36928458
Related structures: EMD-27674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142120 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Soluble cytochrome b562; Volume-regulated anion channel subunit LRRC8A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 911 amino acids
Theoretical weight: 105.53 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q80WG5 (Residues: 1-71, 91-810; Coverage: 98%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: Lrrc8, Lrrc8a, cybC
Sequence domains:
Volume-regulated anion channel subunit LRRC8C Chain: F
Molecule details ›
Chain: F
Length: 813 amino acids
Theoretical weight: 93.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8R502 (Residues: 1-803; Coverage: 100%)
Gene names: Ad158, Fad158, Lrrc8c
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PEE 20 x PEE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-27674
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Physiology of the volume-sensitive/regulatory anion channel VSOR/VRAC. Part 1: from its discovery and phenotype characterization to the molecular entity identification.
Okada Y. (2024)
6 other articles