› 7x4k

Electron Microscopy
3.82Å resolution

Cryo-EM structure of Coxsackievirus B1 empty particle in complex with nAb 9A3 (classified from CVB1 mature virion in complex with 8A10 and 9A3)

Released:
DOI: 10.2210/pdb7x4k/pdb
PDB entry 7x4k coloured by chain, front view.
PDB entry 7x4k coloured by chain, side view.
PDB entry 7x4k coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the synergistic neutralization of coxsackievirus B1 by a triple-antibody cocktail.
Zheng Q, Zhu R, Yin Z, Xu L, Sun H, Yu H, Wu Y, Jiang Y, Huang Q, Huang Y, Zhang D, Liu L, Yang H, He M, Zhou Z, Jiang Y, Chen Z, Zhao H, Que Y, Kong Z, Zhou L, Li T, Zhang J, Luo W, Gu Y, Cheng T, Li S, Xia N
Cell Host Microbe 30 1279-1294.e6 (2022)
PMID: 36002016
Related structures: EMD-33004

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
PDBe Complex ID:
PDB-CPX-224697 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
9A3 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Mus musculus
9A3 light chain Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.47 KDa
Source organism: Mus musculus
VP1 Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.21 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: W8GTF7 (Residues: 1-278; Coverage: 100%)
Sequence domains: picornavirus capsid protein
Genome polyprotein Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 29.12 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 70-332; Coverage: 31%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.33 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: L7UV52 (Residues: 333-570; Coverage: 11%)
Sequence domains: picornavirus capsid protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.82Å
Relevant EMDB volumes: EMD-33004

Quick links

Citations

1 citations in other articles

A counterintuitive antibody cocktail disrupts coxsackievirus.
Zost et al. (2022)