› 7dfs

X-ray diffraction
1.49Å resolution

Crystal structure of a novel 4-O-alpha-L-rhamnosyl-beta-D-glucuronidase from Fusarium oxysporum 12S - Rha-GlcA complex

Released:
DOI: 10.2210/pdb7dfs/pdb
PDB entry 7dfs coloured by chain, front view.
PDB entry 7dfs coloured by chain, side view.
PDB entry 7dfs coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Biochemical and structural characterization of a novel 4-O-α-l-rhamnosyl-β-d-glucuronidase from Fusarium oxysporum.
Kondo T, Kichijo M, Nakaya M, Takenaka S, Arakawa T, Kotake T, Fushinobu S, Sakamoto T
FEBS J 288 4918-4938 (2021)
PMID: 33645879

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Beta-glucuronidase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 50.76 KDa
Source organism: Fusarium oxysporum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A7I8AV45 (Residues: 20-476; Coverage: 100%)
Gene name: FobglcA
Sequence domains: Glycosyl hydrolase family 79 C-terminal beta domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, RAM
Carbohydrate polymer
Carbohydrate polymer : NEW Components: RAM, GCU
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 42.682Å b: 78.567Å c: 127.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.176
Expression system: Komagataella pastoris

Quick links

Citations

2 citation in other articles

FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by silencing a glycosyl hydrolase coding gene expression.
Li et al. (2022)
1 more

1 mention without citation

Biochemical and structural characterization of a novel 4-O-α-l-rhamnosyl-β-d-glucuronidase from Fusarium oxysporum.
Kondo et al. (2021)