› 6t25

Electron Microscopy
3.6Å resolution

Cryo-EM structure of phalloidin-Alexa Flour-546-stabilized F-actin (copolymerized)

Released:
DOI: 10.2210/pdb6t25/pdb
PDB entry 6t25 coloured by chain, front view.
PDB entry 6t25 coloured by chain, side view.
PDB entry 6t25 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments.
Pospich S, Merino F, Raunser S
Structure 28 437-449.e5 (2020)
PMID: 32084355
Related structures: EMD-10367

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
phalloidin Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 5 x ADP
Ligand MG 5 x MG
3 modified residues:
Ligand HIC 5 x HIC
Ligand DTH 5 x DTH
Ligand HYP 5 x HYP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-10367
Expression system: Not provided

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Citations

7 review citations

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
6 more