› 6p95

X-ray diffraction
3.5Å resolution

Structure of Lassa virus glycoprotein in complex with Fab 25.6A

Released:
DOI: 10.2210/pdb6p95/pdb
PDB entry 6p95 coloured by chain, front view.
PDB entry 6p95 coloured by chain, side view.
PDB entry 6p95 coloured by chain, top view.
Source organisms:
Primary publication:
Convergent Structures Illuminate Features for Germline Antibody Binding and Pan-Lassa Virus Neutralization.
Hastie KM, Cross RW, Harkins SS, Zandonatti MA, Koval AP, Heinrich ML, Rowland MM, Robinson JE, Geisbert TW, Garry RF, Branco LM, Saphire EO
Cell 178 1004-1015.e14 (2019)
PMID: 31398326

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231719 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (10 distinct):
Glycoprotein G1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 259 amino acids
Theoretical weight: 29.1 KDa
Source organism: Lassa virus Josiah
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P08669 (Residues: 1-259; Coverage: 53%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
FAB Antibody heavy chain Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 231 amino acids
Theoretical weight: 24.51 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
FAB Antibody light chain Chains: E, G, L
Molecule details ›
Chains: E, G, L
Length: 217 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Glycoprotein G2 Chains: a, b, c
Molecule details ›
Chains: a, b, c
Length: 180 amino acids
Theoretical weight: 20.8 KDa
Source organism: Lassa virus Josiah
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P08669 (Residues: 260-433; Coverage: 35%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6122
Unit cell:
a: 152.242Å b: 152.242Å c: 453.889Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.247
Expression systems:
  • Drosophila melanogaster
  • Cricetulus griseus

Quick links

Citations

5 review citations

Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure.
Hulswit et al. (2021)
4 more

5 mentions without citation

Inter-Lineage Variation of Lassa Virus Glycoprotein Epitopes: A Challenge to Lassa Virus Vaccine Development.
Ibukun FI. (2020)
4 more