› 6kum

X-ray diffraction
1.4Å resolution

Ferredoxin I from C. reinhardtii, low X-ray dose

Released:
DOI: 10.2210/pdb6kum/pdb
PDB entry 6kum coloured by chain, front view.
PDB entry 6kum coloured by chain, side view.
PDB entry 6kum coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
X-ray dose-dependent structural changes of the [2Fe-2S] ferredoxin from Chlamydomonas reinhardtii.
Ohnishi Y, Muraki N, Kiyota D, Okumura H, Baba S, Kawano Y, Kumasaka T, Tanaka H, Kurisu G
J Biochem 167 549-555 (2020)
PMID: 32282907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 9.98 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P07839 (Residues: 32-126; Coverage: 75%)
Gene name: PETF
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain

Ligands and Environments

5 bound ligands:
Ligand FES 2 x FES
Ligand BEN 3 x BEN
Ligand SCN 1 x SCN
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 25.878Å b: 32.326Å c: 49.915Å
α: 72.38° β: 78.06° γ: 72.39°
R-values:
R R work R free
0.172 0.171 0.19
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Changing the tracks: screening for electron transfer proteins to support hydrogen production.
Günzel et al. (2022)
2 more

2 mentions without citation

Effects of Active-Center Reduction of Plant-Type Ferredoxin on Its Structure and Dynamics: Computational Analysis Using Molecular Dynamics Simulations.
Nakayoshi et al. (2022)
1 more