› 5dur

X-ray diffraction
2.82Å resolution

Influenza A virus H5 hemagglutinin globular head in complex with antibody 100F4

Released:
DOI: 10.2210/pdb5dur/pdb
PDB entry 5dur coloured by chain, front view.
PDB entry 5dur coloured by chain, side view.
PDB entry 5dur coloured by chain, top view.
Source organisms:
Primary publication:
Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection.
Zuo T, Sun J, Wang G, Jiang L, Zuo Y, Li D, Shi X, Liu X, Fan S, Ren H, Hu H, Sun L, Zhou B, Liang M, Zhou P, Wang X, Zhang L
OpenAccess logo Nat Commun 6 8855 (2015)
PMID: 26635249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212087 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy Chain of Antibody 100F4 Chains: B, H
Molecule details ›
Chains: B, H
Length: 231 amino acids
Theoretical weight: 24.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Light Chain of Antibody 100F4 Chains: D, L
Molecule details ›
Chains: D, L
Length: 218 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Hemagglutinin Chains: A, C
Molecule details ›
Chains: A, C
Length: 233 amino acids
Theoretical weight: 26.54 KDa
Source organism: Influenza A virus (A/Anhui/1/2005(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q1WDM0 (Residues: 61-284; Coverage: 40%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 88.094Å b: 101.223Å c: 206.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.257
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Influenza Hemagglutinin Structures and Antibody Recognition.
Wu et al. (2020)
3 more

3 mentions without citation

Analysis of the conserved protective epitopes of hemagglutinin on influenza A viruses.
Jiao et al. (2023)
2 more