› 5x20

X-ray diffraction
2.4Å resolution

The ternary structure of D-mandelate dehydrogenase with NADH and anilino(oxo)acetate

Released:
DOI: 10.2210/pdb5x20/pdb
PDB entry 5x20 coloured by chain, front view.
PDB entry 5x20 coloured by chain, side view.
PDB entry 5x20 coloured by chain, top view.
Source organism: Enterococcus faecium DO
Primary publication:
The ternary complex structure of d-mandelate dehydrogenase with NADH and anilino(oxo)acetate.
Furukawa N, Miyanaga A, Nakajima M, Taguchi H
Biochem Biophys Res Commun 486 665-670 (2017)
PMID: 28327357

Function and Biology Details

Reaction catalysed: 
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydropantoate 2-reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 312 amino acids
Theoretical weight: 34.37 KDa
Source organism: Enterococcus faecium DO
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3Y316 (Residues: 1-312; Coverage: 100%)
Gene names: HMPREF0351_10295, panE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 5 x NAD
3 bound ligands:
Ligand AOT 5 x AOT
Ligand GOL 18 x GOL
Ligand SO4 18 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 96.3Å b: 111.95Å c: 108.94Å
α: 90° β: 96.65° γ: 90°
R-values:
R R work R free
0.186 0.184 0.218
Expression system: Escherichia coli

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