› 4ttu

X-ray diffraction
2.18Å resolution

N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose

Released:
DOI: 10.2210/pdb4ttu/pdb
PDB entry 4ttu coloured by chain, front view.
PDB entry 4ttu coloured by chain, side view.
PDB entry 4ttu coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70.
Brison Y, Malbert Y, Czaplicki G, Mourey L, Remaud-Simeon M, Tranier S
J Biol Chem 291 7527-40 (2016)
PMID: 26865636

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1108 amino acids
Theoretical weight: 123.85 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9Q2 (Residues: 1759-2835; Coverage: 39%)
Gene name: dsrE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand NA 1 x NA
Ligand GLC 1 x GLC
Ligand PEG 1 x PEG
2 modified residues:
Ligand TYI 1 x TYI
Ligand IYR 1 x IYR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 68.29Å b: 100.1Å c: 184.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.213
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives.
Graebin et al. (2016)
1 more

2 mentions without citation

Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70.
Brison et al. (2016)
1 more