› 4r8s

X-ray diffraction
1.48Å resolution

Dengue serotype 3 methyltransferase bound to Sinefungin

Released:
DOI: 10.2210/pdb4r8s/pdb
PDB entry 4r8s coloured by chain, front view.
PDB entry 4r8s coloured by chain, side view.
PDB entry 4r8s coloured by chain, top view.
Source organism: Dengue virus 3
Primary publication:
Crystal structure of dengue virus methyltransferase without S-adenosyl-L-methionine.
Noble CG, Li SH, Dong H, Chew SH, Shi PY
Antiviral Res 111 78-81 (2014)
PMID: 25241250

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nonstructural protein NS5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.07 KDa
Source organism: Dengue virus 3
Expression system: Escherichia coli
UniProt:
  • Canonical: C1KBQ3 (Residues: 2491-2752; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SFG 2 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Unit cell:
a: 51.64Å b: 61.14Å c: 183.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.214
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The dengue virus NS5 protein as a target for drug discovery.
Lim et al. (2015)
4 more

5 mentions without citation

Current Trends and Limitations in Dengue Antiviral Research.
Obi et al. (2021)
4 more