› 4g89

X-ray diffraction
2.1Å resolution

Crystal structure of k. pneumoniae mta/adohcy nucleosidase in complex with fragmented s-adenosyl-l-homocysteine

Released:
DOI: 10.2210/pdb4g89/pdb
PDB entry 4g89 coloured by chain, front view.
PDB entry 4g89 coloured by chain, side view.
PDB entry 4g89 coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Entry authors: Norris GE, Brown RL, Anderson BF, Tyler PC, Evans GB

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 25.02 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A6T4W3 (Residues: 1-232; Coverage: 100%)
Gene names: KPN78578_01730, KPN_00174, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand ADE 1 x ADE
Ligand SAH 1 x SAH
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007, null
Spacegroup: C2
Unit cell:
a: 115.346Å b: 59.242Å c: 82.926Å
α: 90° β: 128.46° γ: 90°
R-values:
R R work R free
0.163 0.16 0.22
Expression system: Escherichia coli BL21

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