› 4qf0

X-ray diffraction
6.49Å resolution

Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, quadruple mutant, P21212 form

Released:
DOI: 10.2210/pdb4qf0/pdb
PDB entry 4qf0 coloured by chain, front view.
PDB entry 4qf0 coloured by chain, side view.
PDB entry 4qf0 coloured by chain, top view.
Source organisms:
Entry authors: Lai Y-T, Yeates TO

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein 1; Non-haem bromoperoxidase BPO-A2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 456 amino acids
Theoretical weight: 50.19 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29715 (Residues: 1-278; Coverage: 100%)
  • Canonical: P03485 (Residues: 3-164; Coverage: 64%)
Gene names: M, bpoA2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 175.5Å b: 147.73Å c: 167.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.274 0.271 0.324
Expression system: Escherichia coli BL21(DE3)

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