› 4ivj

X-ray diffraction
7.35Å resolution

Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, I222 form

Released:
DOI: 10.2210/pdb4ivj/pdb
PDB entry 4ivj coloured by chain, front view.
PDB entry 4ivj coloured by chain, side view.
PDB entry 4ivj coloured by chain, top view.
Source organisms:
Primary publication:
Structure and flexibility of nanoscale protein cages designed by symmetric self-assembly.
Lai YT, Tsai KL, Sawaya MR, Asturias FJ, Yeates TO
J Am Chem Soc 135 7738-43 (2013)
PMID: 23621606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein 1; Non-haem bromoperoxidase BPO-A2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 456 amino acids
Theoretical weight: 50.28 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29715 (Residues: 1-278; Coverage: 100%)
  • Canonical: P03485 (Residues: 3-164; Coverage: 64%)
Gene names: M, bpoA2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 129.67Å b: 160.98Å c: 167.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.276 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Engineering protein nanocages as carriers for biomedical applications.
Bhaskar et al. (2017)
13 more

2 mentions without citation

Designing and defining dynamic protein cage nanoassemblies in solution.
Lai et al. (2016)
1 more