› 3vl9

X-ray diffraction
1.2Å resolution

Crystal structure of xeg-xyloglucan

Released:
DOI: 10.2210/pdb3vl9/pdb
PDB entry 3vl9 coloured by chain, front view.
PDB entry 3vl9 coloured by chain, side view.
PDB entry 3vl9 coloured by chain, top view.
Source organism: Aspergillus aculeatus
Primary publication:
Structural basis for inhibition of xyloglucan-specific endo-β-1,4-glucanase (XEG) by XEG-protein inhibitor.
Yoshizawa T, Shimizu T, Hirano H, Sato M, Hashimoto H
J Biol Chem 287 18710-6 (2012)
PMID: 22496365

Function and Biology Details

Reaction catalysed: 
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Xyloglucan-specific endo-beta-1,4-glucanase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 24.18 KDa
Source organism: Aspergillus aculeatus
Expression system: Escherichia coli
UniProt:
  • Canonical: O94218 (Residues: 15-238; Coverage: 100%)
Gene name: xgeA
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYS
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, XYS
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 62.757Å b: 79.283Å c: 80.374Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.127 0.163
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Host Cell Wall Damage during Pathogen Infection: Mechanisms of Perception and Role in Plant-Pathogen Interactions.
Lorrai et al. (2021)
2 more

2 mentions without citation

Structural basis for inhibition of xyloglucan-specific endo-β-1,4-glucanase (XEG) by XEG-protein inhibitor.
Yoshizawa et al. (2012)
1 more