› 3ki9

X-ray diffraction
2.9Å resolution

Crystal structure of Staphylococcus aureus metallopeptidase (Sapep/DapE) in the Mn2+ bound form

Released:
DOI: 10.2210/pdb3ki9/pdb
PDB entry 3ki9 coloured by chain, front view.
PDB entry 3ki9 coloured by chain, side view.
PDB entry 3ki9 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Primary publication:
Crystal structure of Staphylococcus aureus metallopeptidase (Sapep) reveals large domain motions between the manganese-bound and apo-states.
Girish TS, Gopal B
J Biol Chem 285 29406-15 (2010)
PMID: 20610394

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative dipeptidase SACOL1801 Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 55.34 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HF23 (Residues: 1-469; Coverage: 100%)
Gene name: SACOL1801
Sequence domains: Peptidase family M20/M25/M40
Structure domains: Zn peptidases

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: I23
Unit cell:
a: 158.15Å b: 158.15Å c: 158.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.263
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

A mutation in the PP2C phosphatase gene in a Staphylococcus aureus USA300 clinical isolate with reduced susceptibility to vancomycin and daptomycin.
Passalacqua et al. (2012)
6 more

5 mentions without citation

Crystal structure of Staphylococcus aureus metallopeptidase (Sapep) reveals large domain motions between the manganese-bound and apo-states.
Girish et al. (2010)
4 more