› 2qha

X-ray diffraction
1.45Å resolution

From Structure to Function: Insights into the Catalytic Substrate Specificity and Thermostability Displayed by Bacillus subtilis mannanase BCman

Released:
DOI: 10.2210/pdb2qha/pdb
PDB entry 2qha coloured by chain, front view.
PDB entry 2qha coloured by chain, side view.
PDB entry 2qha coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
From structure to function: insights into the catalytic substrate specificity and thermostability displayed by Bacillus subtilis mannanase BCman.
Yan XX, An XM, Gui LL, Liang DC
J Mol Biol 379 535-44 (2008)
PMID: 18455734

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 38.96 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5PSP8 (Residues: 27-362; Coverage: 99%)
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 45.179Å b: 58.785Å c: 63.463Å
α: 83.03° β: 82.42° γ: 77.61°
R-values:
R R work R free
0.16 0.158 0.19
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
4 more

6 mentions without citation

Structural and biochemical analyses of glycoside hydrolase families 5 and 26 β-(1,4)-mannanases from Podospora anserina reveal differences upon manno-oligosaccharide catalysis.
Couturier et al. (2013)
5 more