› 2hqm

X-ray diffraction
2.4Å resolution

Crystal Structure of Glutathione Reductase Glr1 from the Yeast Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb2hqm/pdb
PDB entry 2hqm coloured by chain, front view.
PDB entry 2hqm coloured by chain, side view.
PDB entry 2hqm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of glutathione reductase Glr1 from the yeast Saccharomyces cerevisiae.
Yu J, Zhou CZ
Proteins 68 972-9 (2007)
PMID: 17554778

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 53.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P41921 (Residues: 16-483; Coverage: 97%)
Gene names: GLR1, LPG17W, YPL091W
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH

Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand NAG 2 x NAG
Ligand PO4 2 x PO4
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P42
Unit cell:
a: 116.966Å b: 116.966Å c: 85.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.205
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
3 more

8 mentions without citation

Contribution of Fdh3 and Glr1 to Glutathione Redox State, Stress Adaptation and Virulence in Candida albicans.
Tillmann et al. (2015)
7 more