› 1nwk

X-ray diffraction
1.85Å resolution

CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE

Released:
DOI: 10.2210/pdb1nwk/pdb
PDB entry 1nwk coloured by chain, front view.
PDB entry 1nwk coloured by chain, side view.
PDB entry 1nwk coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics.
Graceffa P, Dominguez R
J Biol Chem 278 34172-80 (2003)
PMID: 12813032

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand ANP 1 x ANP
Ligand RHO 1 x RHO
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 112.216Å b: 37.368Å c: 85.027Å
α: 90° β: 108.13° γ: 90°
R-values:
R R work R free
0.187 0.187 0.226

Quick links

Citations

17 review citations

Actin structure and function.
Dominguez et al. (2011)
16 more

40 mentions without citation

Altered Smooth Muscle Cell Force Generation as a Driver of Thoracic Aortic Aneurysms and Dissections.
Milewicz et al. (2017)
39 more