PDBe 1kc2

X-ray diffraction
2.1Å resolution

structure of the triple (Lys(beta)D3Ala, Asp(beta)C8Ala, AspCD2Ala) mutant of the Src SH2 domain bound to the PQpYEEIPI peptide


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PQpYEEIPI peptide Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.07 KDa
Source organism: Synthetic construct
Expression system: Not provided
Tyrosine-protein kinase transforming protein Src Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 11.7 KDa
Source organism: Rous sarcoma virus
Expression system: Escherichia coli
  • Canonical: P00524 (Residues: 145-247; Coverage: 20%)
Gene name: V-SRC
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 62.447Å b: 62.447Å c: 133.68Å
α: 90° β: 90° γ: 90°
R R work R free
0.229 0.229 0.267
Expression systems:
  • Not provided
  • Escherichia coli