PDBe 1s9c

X-ray diffraction
3Å resolution

Crystal structure analysis of the 2-enoyl-CoA hydratase 2 domain of human peroxisomal multifunctional enzyme type 2

Released:

Function and Biology Details

Reactions catalysed:
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O. 
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl- CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl- CoA + H(2)O. 
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 298 amino acids
Theoretical weight: 32.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51659 (Residues: 318-615; Coverage: 41%)
Gene names: EDH17B4, HSD17B4, SDR8C1
Sequence domains:
Structure domains: Thiol Ester Dehydrase; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11, EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 98.295Å b: 105.431Å c: 206.944Å
α: 90° β: 103.37° γ: 90°
R-values:
R R work R free
0.228 0.228 0.265
Expression system: Escherichia coli BL21(DE3)