› 5l0h

X-ray diffraction
2.9Å resolution

Human metavinculin MVt cardiomyopathy-associated mutant R975W (residues 959-1134) in complex with PIP2

Released:
DOI: 10.2210/pdb5l0h/pdb
PDB entry 5l0h coloured by chain, front view.
PDB entry 5l0h coloured by chain, side view.
PDB entry 5l0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization.
Chinthalapudi K, Rangarajan ES, Brown DT, Izard T
Proc Natl Acad Sci U S A 113 9539-44 (2016)
PMID: 27503891

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 19.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18206 (Residues: 959-1134; Coverage: 16%)
Gene name: VCL
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like

Ligands and Environments

3 bound ligands:
Ligand PEG 1 x PEG
Ligand PIO 1 x PIO
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6422
Unit cell:
a: 80.951Å b: 80.951Å c: 131.762Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.255 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Roles of Membrane Domains in Integrin-Mediated Cell Adhesion.
Lietha et al. (2020)
1 more

1 mention without citation

Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization.
Chinthalapudi et al. (2016)