› 5h9w

X-ray diffraction
2.6Å resolution

Crystal structure of Regnase PIN domain, form II

Released:
DOI: 10.2210/pdb5h9w/pdb
PDB entry 5h9w coloured by chain, front view.
PDB entry 5h9w coloured by chain, side view.
PDB entry 5h9w coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the regulation of enzymatic activity of Regnase-1 by domain-domain interactions.
Yokogawa M, Tsushima T, Noda NN, Kumeta H, Enokizono Y, Yamashita K, Standley DM, Takeuchi O, Akira S, Inagaki F
Sci Rep 6 22324 (2016)
PMID: 26927947

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease ZC3H12A Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5D1E7 (Residues: 134-339; Coverage: 35%)
Gene names: Mcpip, Mcpip1, Zc3h12a
Sequence domains: Zc3h12a-like Ribonuclease NYN domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3121
Unit cell:
a: 113.333Å b: 113.333Å c: 78.373Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.231
Expression system: Escherichia coli

Quick links

Citations

13 review citations

RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins.
Fu et al. (2017)
12 more

1 mention without citation

Structural basis for the regulation of enzymatic activity of Regnase-1 by domain-domain interactions.
Yokogawa et al. (2016)