› 5h7c

X-ray diffraction
2.7Å resolution

Crystal structure of a repeat protein with two Protein A-DHR14 repeat modules

Released:
DOI: 10.2210/pdb5h7c/pdb
PDB entry 5h7c coloured by chain, front view.
PDB entry 5h7c coloured by chain, side view.
PDB entry 5h7c coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A Chains: A, C
Molecule details ›
Chains: A, C
Length: 404 amino acids
Theoretical weight: 45.37 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P38507 (Residues: 38-138, 139-196, 197-200, 201-218, 219-324; Coverage: 60%)
Gene name: spa
Sequence domains: B domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 48.658Å b: 75.528Å c: 80.013Å
α: 84.19° β: 83.72° γ: 75.21°
R-values:
R R work R free
0.206 0.202 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles