› 5a9x

X-ray diffraction
3.8Å resolution

Structure of GDP bound BipA

Released:
DOI: 10.2210/pdb5a9x/pdb
PDB entry 5a9x coloured by chain, front view.
PDB entry 5a9x coloured by chain, side view.
PDB entry 5a9x coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of BipA in GTP form bound to the ratcheted ribosome.
Kumar V, Chen Y, Ero R, Ahmed T, Tan J, Li Z, Wong AS, Bhushan S, Gao YG
Proc Natl Acad Sci U S A 112 10944-9 (2015)
PMID: 26283392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit assembly factor BipA Chain: A
Molecule details ›
Chain: A
Length: 607 amino acids
Theoretical weight: 67.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3B2 (Residues: 1-607; Coverage: 100%)
Gene names: E2348C_4177, bipA, o591, typA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4132
Unit cell:
a: 241.946Å b: 241.946Å c: 241.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.248 0.313
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The magic dance of the alarmones (p)ppGpp.
Steinchen et al. (2016)
8 more

1 mention without citation

Structure of BipA in GTP form bound to the ratcheted ribosome.
Kumar et al. (2015)