› 5j8l

X-ray diffraction
1.73Å resolution

Crystal structure of D-tagatose 3-epimerase C66S from Pseudomonas cichorii in complex with 1-deoxy L-tagatose, using a crystal grown in microgravity

Released:
DOI: 10.2210/pdb5j8l/pdb
PDB entry 5j8l coloured by chain, front view.
PDB entry 5j8l coloured by chain, side view.
PDB entry 5j8l coloured by chain, top view.
Source organism: Pseudomonas cichorii
Primary publication:
X-ray structures of the Pseudomonas cichorii D-tagatose 3-epimerase mutant form C66S recognizing deoxy sugars as substrates.
Yoshida H, Yoshihara A, Ishii T, Izumori K, Kamitori S
Appl Microbiol Biotechnol 100 10403-10415 (2016)
PMID: 27368739

Function and Biology Details

Reaction catalysed: 
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-tagatose 3-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 300 amino acids
Theoretical weight: 33.86 KDa
Source organism: Pseudomonas cichorii
Expression system: Escherichia coli
UniProt:
  • Canonical: O50580 (Residues: 1-290; Coverage: 100%)
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand TGJ 2 x TGJ
Ligand TGK 8 x TGK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 52.58Å b: 126.535Å c: 98.903Å
α: 90° β: 101.45° γ: 90°
R-values:
R R work R free
0.197 0.194 0.219
Expression system: Escherichia coli

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Citations

2 review citations

D-Tagatose Is a Promising Sweetener to Control Glycaemia: A New Functional Food.
Guerrero-Wyss et al. (2018)
1 more