› 5e1q

X-ray diffraction
1.94Å resolution

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Retaining alpha-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 74.35 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A6L0 (Residues: 27-662; Coverage: 99%)
Gene name: BT_1871
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLA, GAL
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 60.62Å b: 100.624Å c: 237.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Synthesis of Glycosides by Glycosynthases.
Hayes et al. (2017)
1 more