› 5dem

X-ray diffraction
1.81Å resolution

Structure of Pseudomonas aeruginosa LpxA

Released:
DOI: 10.2210/pdb5dem/pdb
PDB entry 5dem coloured by chain, front view.
PDB entry 5dem coloured by chain, side view.
PDB entry 5dem coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PA7
Primary publication:
Structures of Pseudomonas aeruginosa LpxA Reveal the Basis for Its Substrate Selectivity.
Smith EW, Zhang X, Behzadi C, Andrews LD, Cohen F, Chen Y
Biochemistry 54 5937-48 (2015)
PMID: 26352800

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 258 amino acids
Theoretical weight: 28.05 KDa
Source organism: Pseudomonas aeruginosa PA7
Expression system: Escherichia coli
UniProt:
  • Canonical: A6V1E4 (Residues: 1-258; Coverage: 100%)
Gene names: PSPA7_1495, lpxA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 79.031Å b: 82.176Å c: 220.515Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.211
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, inhibition, and regulation of essential lipid A enzymes.
Zhou et al. (2017)
2 more

3 mentions without citation

Discovery of dual-activity small-molecule ligands of Pseudomonas aeruginosa LpxA and LpxD using SPR and X-ray crystallography.
Kroeck et al. (2019)
2 more