4xqg

X-ray diffraction
1.42Å resolution

Crystal Structure of the Homospermidine Synthase (HSS) variant E237Q from Blastochloris viridis in Complex with NAD.

Released:

Function and Biology Details

Reaction catalysed:
2 putrescine = sym-homospermidine + NH(3)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-128445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homospermidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 52.59 KDa
Source organism: Blastochloris viridis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32323 (Residues: 3-477; Coverage: 100%)
Gene name: hss
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 59.809Å b: 109.25Å c: 157.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.129 0.161
Expression system: Escherichia coli BL21(DE3)