› 4m9u

X-ray diffraction
1.6Å resolution

The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4

Released:
DOI: 10.2210/pdb4m9u/pdb
PDB entry 4m9u coloured by chain, front view.
PDB entry 4m9u coloured by chain, side view.
PDB entry 4m9u coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Tan K, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthase Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 41.39 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NGE8 (Residues: 1-365; Coverage: 100%)
Gene names: FTT_0897, purK
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand ACT 10 x ACT
Ligand FMT 1 x FMT
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 85.313Å b: 85.313Å c: 107.673Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.196
Expression system: Escherichia coli BL21(DE3)

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