› 4fxy

X-ray diffraction
2.8Å resolution

Crystal structure of rat neurolysin with bound pyrazolidin inhibitor

Released:
DOI: 10.2210/pdb4fxy/pdb
PDB entry 4fxy coloured by chain, front view.
PDB entry 4fxy coloured by chain, side view.
PDB entry 4fxy coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Allosteric inhibition of the neuropeptidase neurolysin.
Hines CS, Ray K, Schmidt JJ, Xiong F, Feenstra RW, Pras-Raves M, de Moes JP, Lange JH, Melikishvili M, Fried MG, Mortenson P, Charlton M, Patel Y, Courtney SM, Kruse CG, Rodgers DW
J Biol Chem 289 35605-19 (2014)
PMID: 25378390

Function and Biology Details

Reaction catalysed: 
Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurolysin, mitochondrial Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 693 amino acids
Theoretical weight: 79.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P42676 (Residues: 24-704; Coverage: 97%)
Gene name: Nln
Sequence domains: Peptidase family M3
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 0W2 2 x 0W2
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 88Å b: 131.4Å c: 144.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.235 0.287
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Adaptability of protein structures to enable functional interactions and evolutionary implications.
Haliloglu et al. (2015)
3 more

2 mentions without citation

Allosteric inhibition of the neuropeptidase neurolysin.
Hines et al. (2014)
1 more