› 4ywa

X-ray diffraction
1.19Å resolution

Structural Insight into Divalent Galactoside Binding to Pseudomonas aeruginosa lectin LecA

Released:
DOI: 10.2210/pdb4ywa/pdb
PDB entry 4ywa coloured by chain, front view.
PDB entry 4ywa coloured by chain, side view.
PDB entry 4ywa coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural Insight into Multivalent Galactoside Binding to Pseudomonas aeruginosa Lectin LecA.
Visini R, Jin X, Bergmann M, Michaud G, Pertici F, Fu O, Pukin A, Branson TR, Thies-Weesie DM, Kemmink J, Gillon E, Imberty A, Stocker A, Darbre T, Pieters RJ, Reymond JL
ACS Chem Biol 10 2455-62 (2015)
PMID: 26295304

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA-I galactophilic lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand 4J9 2 x 4J9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 40.77Å b: 72.32Å c: 78.71Å
α: 117.23° β: 105° γ: 89.94°
R-values:
R R work R free
0.187 0.186 0.204
Expression system: Escherichia coli

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Citations

6 review citations

From Nanobiotechnology, Positively Charged Biomimetic Dendrimers as Novel Antibacterial Agents: A Review.
Alfei et al. (2020)
5 more

4 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
3 more