› 4f4b

X-ray diffraction
1.87Å resolution

Structure of OSH4 with a cholesterol analog

Released:
DOI: 10.2210/pdb4f4b/pdb
PDB entry 4f4b coloured by chain, front view.
PDB entry 4f4b coloured by chain, side view.
PDB entry 4f4b coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
Koag MC, Cheun Y, Kou Y, Ouzon-Shubeita H, Min K, Monzingo AF, Lee S
Steroids 78 938-44 (2013)
PMID: 23756172

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 49.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P35844 (Residues: 2-434; Coverage: 100%)
Gene names: BSR3, KES1, LPI3C, OSH4, P2614, YPL145C
Sequence domains: Oxysterol-binding protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand L39 2 x L39
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P1
Unit cell:
a: 51.123Å b: 65.397Å c: 82.314Å
α: 96.56° β: 100.28° γ: 95.91°
R-values:
R R work R free
0.217 0.214 0.271
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Molecular basis for sterol transport by StART-like lipid transfer domains.
Horenkamp et al. (2018)
2 more