› 3l6j

X-ray diffraction
2.3Å resolution

Structure of cinaciguat (bay 58-2667) bound to nostoc H-NOX domain

Released:
DOI: 10.2210/pdb3l6j/pdb
PDB entry 3l6j coloured by chain, front view.
PDB entry 3l6j coloured by chain, side view.
PDB entry 3l6j coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Structure of cinaciguat (BAY 58-2667) bound to Nostoc H-NOX domain reveals insights into heme-mimetic activation of the soluble guanylyl cyclase.
Martin F, Baskaran P, Ma X, Dunten PW, Schaefer M, Stasch JP, Beuve A, van den Akker F
J Biol Chem 285 22651-7 (2010)
PMID: 20463019

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-187121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.21 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-189; Coverage: 100%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

1 bound ligand:
Ligand Z90 2 x Z90
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P213
Unit cell:
a: 123.268Å b: 123.268Å c: 123.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.193
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Soluble guanylate cyclase as an emerging therapeutic target in cardiopulmonary disease.
Stasch et al. (2011)
20 more

6 mentions without citation

Structure and Activation of Soluble Guanylyl Cyclase, the Nitric Oxide Sensor.
Montfort et al. (2017)
5 more