› 3gxw

X-ray diffraction
1.9Å resolution

Structure of the SH2 domain of the Candida glabrata transcription elongation factor Spt6, crystal form A

Released:
DOI: 10.2210/pdb3gxw/pdb
PDB entry 3gxw coloured by chain, front view.
PDB entry 3gxw coloured by chain, side view.
PDB entry 3gxw coloured by chain, top view.
Source organism: [Candida] glabrata
Primary publication:
Structure and in vivo requirement of the yeast Spt6 SH2 domain.
Dengl S, Mayer A, Sun M, Cramer P
J Mol Biol 389 211-25 (2009)
PMID: 19371747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor SPT6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 103 amino acids
Theoretical weight: 12.43 KDa
Source organism: [Candida] glabrata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6FLB1 (Residues: 1250-1348; Coverage: 7%)
Gene names: CAGL0L04774g, SPT6
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

2 bound ligands:
Ligand SIN 2 x SIN
Ligand NA 4 x NA
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P65
Unit cell:
a: 54.499Å b: 54.499Å c: 253.434Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.241
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Chromatin and transcription in yeast.
Rando et al. (2012)
5 more

3 mentions without citation

Loops govern SH2 domain specificity by controlling access to binding pockets.
Kaneko et al. (2010)
2 more