Transcription elongation factor Spt6 (IPR017072)

Short name: TF_Spt6

Overlapping homologous superfamilies


Family relationships



This family consists of several eukaryotic nuclear transcription elongation proteins characterised by an extremely acidic N-terminal region. In yeast, increased or decreased dosage of Spt6 protein can change gene expression [PMID: 8786132]. In addition, all members contain a YqgFc domain. This domain is found primarily in low-GC Gram-positive bacteria Holliday junction resolvases. However, in Spt6 orthologues, the catalytic residues are substituted, indicating that they lack resolvase activity. Some members contain a copy of the S1 domain (IPR003029) that occurs in a wide range of RNA associated proteins. It is structurally similar to cold shock protein, which binds nucleic acids. The S1 domain has an OB-fold structure. Adjacent to this domain one to two copies of the SH2 domain are present (IPR000980) which are regulatory modules of signal cascades.

Three transcription-elongation factors--Spt4, Spt5, and Spt6--are conserved among eukaryotes and are essential for transcription via modulation of chromatin structure. Spt4 and Spt5 are tightly associated in a complex, while the physical association of Spt6 is considerably weaker. It has been demonstrated that Spt4, Spt5, and Spt6 play roles in transcription elongation in both yeast and humans, including a role in activation by Tat. It is known that Spt4, Spt5, and Spt6 are general transcription-elongation factors, controlling transcription both positively and negatively in important regulatory and developmental roles [PMID: 11182892].

GO terms

Biological Process

GO:0032784 regulation of DNA-templated transcription, elongation
GO:0006357 regulation of transcription by RNA polymerase II

Molecular Function

GO:0000991 transcription factor activity, core RNA polymerase II binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.