› 3tbe

X-ray diffraction
2.85Å resolution

The crystal structure of the complex of Streptococcus agalactiae sortase C1 and MTSET

Released:
DOI: 10.2210/pdb3tbe/pdb
PDB entry 3tbe coloured by chain, front view.
PDB entry 3tbe coloured by chain, side view.
PDB entry 3tbe coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Primary publication:
The crystal structure analysis of group B Streptococcus sortase C1: a model for the "lid" movement upon substrate binding.
Khare B, Fu ZQ, Huang IH, Ton-That H, Narayana SV
J Mol Biol 414 563-77 (2011)
PMID: 22033482

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sortase family protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 230 amino acids
Theoretical weight: 25.78 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E0S7 (Residues: 43-260; Coverage: 72%)
Gene name: SAG0647
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

3 bound ligands:
Ligand ETM 6 x ETM
Ligand SO4 5 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 122.41Å b: 70.65Å c: 194.44Å
α: 90° β: 90.43° γ: 90°
R-values:
R R work R free
0.253 0.25 0.296
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular features of the sortase enzyme family.
Bradshaw et al. (2015)
1 more

4 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
3 more