3p27

X-ray diffraction
2.95Å resolution

Crystal structure of S. cerevisiae Hbs1 protein (GDP-bound form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-152443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 1 alpha-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 483 amino acids
Theoretical weight: 54.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32769 (Residues: 135-611; Coverage: 78%)
Gene names: HBS1, YKR084C, YKR404
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P42212
Unit cell:
a: 109.964Å b: 109.964Å c: 188.199Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.278
Expression system: Escherichia coli